Human Osteoblasts Attachment to Guided Tissue Regeneration Membranes Which Were Coated Either With Platelet-Rich Plasma or Platelet-Poor Plasma

Objective: To determine whether human alveolar bone osteoblasts (HABO) attachment to commercial available guided tissue regeneration (GTR) membranes can be enhanced by coating with freshly prepared human platelet-rich plasma (PRP) and platelet poor plasma (PPP).

Methods: Human osteoblasts established from tissue explants were used at 4 th passage in culture. Human whole blood from healthy subjects was collected and centrifuged twice to produce the PRP fraction and PPP fraction. Double-sided adhesive tape was used to fix 3 mm discs of each membrane and cover-slides to the bottom of a 24-well tissue culture plate. A (GoreTex-ePTFE), B (GoreTex-Resolut) and C (Inion-GTR) membranes were studied. Cover-slides were positive control. Membranes or cover-slides were exposed to PRP, PPP or PBS respectively for 2 hours. Membranes and cover-slides were seeded with osteoblasts (5 x 10(7) cells/L) and allowed to attach for 24 hours. After staining with hematoxylin, the number of attached cells per mm(2) was counted using a light microscope with graticule. The the ultrastructure of osteoblasts attachment to the membranes was observed by scanningelectronic microscopy.

Results: PRP and PPP-treated membranes significantly enhanced osteoblasts attachment compared to PBS-treated membranes (P < 0.05). There was more osteoblasts attachment in the PRP-treated membranes than in the PPP-treated membranes (P < 0.05). Cover-slides showed more osteoblasts attachment than the three membranes (P < 0.05). B and C membranes showed higher cell attachment than A membranes (P < 0.05). SEM showed that osteoblasts attached to the membranes treated by PRP were spindle and stretched well, and there were platelets, fibrins in a interlaced mesh on the membranes, which appeared to grow in a multiplayer style. The osteoblasts attached to the membranes treated by PPP or PBS were round and partially attached.

Conclusions: PRP and PPP could improve attachment of osteoblasts in the three membranes, and PRP altered and enhanced the way of the attachment to the membranes.