Platelet-Rich Plasma Induces mRNA Expression of VEGF and PDGF in Rat Bone Marrow Stromal Cell Differentiation

Objective: To investigate the potentially useful of platelet-rich plasma (PRP) on mRNA expression of angiogenesis.

Methods: Adjunct assay and reverse-transcription polymerase chain reaction (RT-PCR) analysis of type I collagen, vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) in rat bone marrow stromal cells differentiation in 14 days’ culture.

Results: The PRP significantly elevated alkaline phosphatase activity after day 5 (P < .05), and DNA and protein content increased at culture days 1, 3, and 5 (P < .01) with PRP compared with control. The RT-PCR demonstrated that type I collagen was expressed in all subtrates and remained high with PRP during 14 days of culture, and that mRNA expression of VEGF and PDGF were higher over time.

Conclusions: This study indicates a potential contribution of PRP as possibly starting the process of angiogenesis, recruiting the endothelial cells which line blood vessels, and beginning the initiation of bone regeneration.